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目錄號

尺寸

A-1018-010

10微克

A-1018-050

50微克

A-1018-100

100微克

應用：

DB，ELISA，IF，IHC，MeDIP

研究領域：

DNA甲基化

克隆：

單克隆

共軛：

非結合

主辦：

老鼠

同種型：

的IgG1

純化：

親和純化

反應：

范圍廣泛

100％保證：

6個月

背景 
技術已經檢測到5-羥甲基胞嘧啶（5-hmC）作為在轉錄調節中具有功能的第六DNA堿基在人和小鼠腦和胚胎干（ES）細胞中是豐富的。在哺乳動物中，它可以通過 5-甲基胞嘧啶（5-mC）的氧化產生， 5-甲基胞嘧啶是由10-mC羥化酶的10-eleven易位（TET）家族介導的反應。5-hmC在DNA甲基化，染色質重塑和基因表達調控中發揮了比5-mC更重要和不同的作用。

濃度 
1毫克/毫升

描述 
小鼠單克隆抗體5-羥甲基胞嘧啶（5-hmC），克隆HMC / 4D9

特異性 
修飾的堿基5-羥甲基胞嘧啶（5-hmC），種類繁多

同種型 
IgG1

配方 
10mM磷酸鹽緩沖液，150mM NaCl，pH 7.4 

儲存  4°C。對于長期儲存，等分并儲存在-20°C。避免反復凍融。多次冷凍/解凍循環可能導致性能下降。自發貨之日起6個月內穩定。

純度 
蛋白A純化

處理建議 
為了大限度地回收產品，請在打開蓋子之前對小瓶進行離心

替代名稱 
5-羥甲基胞苷，抗5-羥甲基胞苷，抗5-羥甲基胞嘧啶，抗-5hmC，抗-5-hmC，抗-5-hmeC，抗5hmeC，5hmC，5hmeC，5-hmeC

應用 
Immunofluorescence：1：200 - 1：500 
Immunohistochemistry：1：200 - 1：500 
ELISA：1：1000 - 1：2000 
Dot Blot：1：2000 
hMeDIP：0.5-1μg/ 10 ⁶細胞

圖1. 含有胞嘧啶（C），5-甲基胞嘧啶（5mC）或5-羥甲基胞嘧啶（5hmC）的DNA寡核苷酸的A-1018的斑點印跡分析。將10ng C，5mC和5hmC DNA寡核苷酸各自點在膜上。將膜與0.5μg/ ml的Epigentek的5hmC克隆HMC / 4D9抗體（1：2000稀釋）一起溫育，然后與過氧化物酶綴合的山羊抗小鼠IgG（稀釋度1：3000）一起溫育。僅用5-hmC DNA寡核苷酸觀察到特異性信號。


圖2. Epigentek的5-hmC克隆HMC / 4D9抗體對5-hmC DNA的高靈敏度和特異性的證明。分別將含有5-hmC，5-mC或僅含有胞嘧啶的合成DNA寡核苷酸以不同濃度添加到測定孔中，然后僅用5-hmC DNA檢測特異性信號。

圖3.  羥甲基化DNA的選擇性富集。將50μg未甲基化，甲基化和羥甲基化的DNA寡核苷酸分別摻入片段化的人基因組DNA（500ng）中。用Epigentek的5-hmC克隆HMC / 4D9抗體處理hMeDIP。用引物通過實時PCR分析洗脫的DNA，以檢測摻入的DNA寡核苷酸的存在。倍數富集代表回收的DNA寡核苷酸的量，并且基于Cts計算。

Cat. #

Antibody Panel Pack

Modifications

Sizes Each

C10000

 Histone H3 Methylation Antibody Panel Pack I- Active Genes

25 µg

C10001

 Histone H3 Methylation Antibody Panel Pack I - Repression Genes

25 µg

C10002

 Histone H3 Methylation Antibody Panel Pack II - Active Genes

25 µg

C10003

 Histone H3 Methylation Antibody Panel Pack II - Repression Genes

25 µg

C10004

 Histone H3 Methylation Antibody Panel Pack III - Active Genes

25 µg

C10005

 Histone H3K4 Methylation Antibody Panel Pack

25 µg

C10006

 Histone H3K9 Methylation Antibody Panel Pack

25 µg

C10007

 Histone H3K27 Methylation Antibody Panel Pack

25 µg

C10008

 Histone H3K36 Methylation Antibody Panel Pack

25 µg

C10009

 Histone H3K79 Methylation Antibody Panel Pack

25 µg

C10010

 Histone H3 Acetylation Antibody Panel Pack I

25 µg

C10011

 Histone H3 Acetylation Antibody Panel Pack II

25 µg

C10012

 Histone H4K20 Methylation Antibody Panel Pack

25 µg

C10013

 Histone H4 Acetylation Antibody Panel Pack

25 µg

C10014

 Histone H3 Phosphorylation Antibody Panel Pack

25 µg

C10015

 Histone H3R2 Methylation Antibody Panel Pack

25 µg

C10016

 Histone H3R8 Methylation Antibody Panel Pack

25 µg

C10017

 Histone H3R17 Methylation Antibody Panel Pack

25 µg

C10018

 Histone H3R26 Methylation Antibody Panel Pack

25 µg

C10019

 Histone H4R3 Methylation Antibody Panel Pack

25 µg

Image

Description

Cat. No.

Size

Price

Application:

DB, ELISA, IF, IHC, MeDIP

Areas of Research:

DNA Methylation

Clonality:

Monoclonal

Conjugate:

Unconjugated

Host:

Mouse

Isotype:

IgG1

Purification:

Affinity Purified

Reactivity:

Broad Range

A-1018-010

10 µg

A-1018-050

50 µg

A-1018-100

100 µg

Application:

DB, ELISA, IF, IHC, IP, MeDIP

Areas of Research:

DNA Methylation

Clonality:

Monoclonal

Conjugate:

Unconjugated

Host:

Mouse

Isotype:

IgG1

Purification:

Affinity Purified

Reactivity:

Broad Range

A-1014-010

10 µg

A-1014-050

50 µg

A-1014-100

100 µg

Application:

WB

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

Unconjugated

Host:

Rabbit

Isotype:

IgG

Purification:

Affinity Purified

Reactivity:

Human, Mouse, Rat

A-2723-050

50 µg

A-2723-100

100 µg

Application:

ELISA, IF, IHC

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

Unconjugated

Host:

Rabbit

Isotype:

IgG

Purification:

>95%, Protein G Purified

Reactivity:

Human

Trial Size Available:

Yes

A57365-020

20 µg

A57365-050

50 µg

A57365-100

100 µg

Application:

ELISA

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

Biotin

Host:

Rabbit

Isotype:

IgG

Purification:

>95%, Protein G Purified

Reactivity:

Human

A57368-050

50 µg

A57368-100

100 µg

Application:

ELISA

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

FITC

Host:

Rabbit

Isotype:

IgG

Purification:

>95%, Protein G Purified

Reactivity:

Human

A57367-050

50 µg

A57367-100

100 µg

Application:

ELISA

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

HRP

Host:

Rabbit

Isotype:

IgG

Purification:

>95%, Protein G Purified

Reactivity:

Human

A57366-050

50 µg

A57366-100

100 µg

Application:

ELISA

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

Unconjugated

Host:

Rabbit

Isotype:

IgG

Purification:

>95%, Protein G Purified

Reactivity:

Acetobacter pasteurianus

Trial Size Available:

Yes

A50869-020

20 µg

A50869-050

50 µg

A50869-100

100 µg

Application:

ELISA

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

Biotin

Host:

Rabbit

Isotype:

IgG

Purification:

>95%, Protein G Purified

Reactivity:

Acetobacter pasteurianus

A50872-050

50 µg

A50872-100

100 µg

Application:

ELISA

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

FITC

Host:

Rabbit

Isotype:

IgG

Purification:

>95%, Protein G Purified

Reactivity:

Acetobacter pasteurianus

A50871-050

50 µg

A50871-100

100 µg

Application:

ELISA

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

HRP

Host:

Rabbit

Isotype:

IgG

Purification:

>95%, Protein G Purified

Reactivity:

Acetobacter pasteurianus

A50870-050

50 µg

A50870-100

100 µg

Application:

ELISA

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

Unconjugated

Host:

Rabbit

Isotype:

IgG

Purification:

>95%, Protein G Purified

Reactivity:

Human

Trial Size Available:

Yes

A58106-020

20 µg

A58106-050

50 µg

A58106-100

100 µg

Application:

ELISA

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

Biotin

Host:

Rabbit

Isotype:

IgG

Purification:

>95%, Protein G Purified

Reactivity:

Human

A58107-050

50 µg

A58107-100

100 µg

Application:

ELISA

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

FITC

Host:

Rabbit

Isotype:

IgG

Purification:

>95%, Protein G Purified

Reactivity:

Human

A58108-050

50 µg

A58108-100

100 µg

Application:

ELISA

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

HRP

Host:

Rabbit

Isotype:

IgG

Purification:

>95%, Protein G Purified

Reactivity:

Human

A58109-050

50 µg

A58109-100

100 µg

Application:

ELISA, IF, IP, WB

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

Unconjugated

Host:

Rabbit

Isotype:

IgG

Purification:

Affinity Purified

Reactivity:

Human, Mouse, Rat

A-1013-050

50 µg

A-1013-100

100 µg

Application:

ELISA, IHC

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

Unconjugated

Host:

Rabbit

Isotype:

IgG

Purification:

>95%, Protein G Purified

Reactivity:

Human

Trial Size Available:

Yes

A68018-020

20 µg

A68018-050

50 µg

A68018-100

100 µg

Application:

ELISA

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

Biotin

Host:

Rabbit

Isotype:

IgG

Purification:

>95%, Protein G Purified

Reactivity:

Human

A68021-050

50 µg

A68021-100

100 µg

Application:

ELISA

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

FITC

Host:

Rabbit

Isotype:

IgG

Purification:

>95%, Protein G Purified

Reactivity:

Human

A68020-050

50 µg

A68020-100

100 µg

Application:

ELISA

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

HRP

Host:

Rabbit

Isotype:

IgG

Purification:

>95%, Protein G Purified

Reactivity:

Human

A68019-050

50 µg

A68019-100

100 µg

Application:

ELISA, IF, IP, WB

Areas of Research:

DNA Methylation

Clonality:

Monoclonal

Conjugate:

Unconjugated

Host:

Mouse

Isotype:

IgG1

Purification:

Affinity Purified

Reactivity:

Human, Mouse

A-1001-050

50 µg

A-1001-100

100 µg

Application:

ChIP, IF, IHC, IP, WB

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

Unconjugated

Host:

Rabbit

Isotype:

IgG

Purification:

Affinity Purified

Reactivity:

Human, Mouse, Rat

A-1700-050

50 µg

A-1700-100

100 µg

Application:

IHC, WB

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

Unconjugated

Host:

Rabbit

Isotype:

IgG

Purification:

Affinity Purified

Reactivity:

Human, Mouse

A-1002-050

50 µl

A-1002-100

100 µl

Application:

IF, IHC, WB

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

Unconjugated

Host:

Rabbit

Isotype:

IgG

Purification:

Affinity Purified

Reactivity:

Human, Mouse, Rat

A-1003-050

50 µg

A-1003-100

100 µg

Application:

ELISA, IF, IHC

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

Unconjugated

Host:

Rabbit

Isotype:

IgG

Purification:

>95%, Protein G Purified

Reactivity:

Human, Mouse

Trial Size Available:

Yes

A52569-020

20 µg

A52569-050

50 µg

A52569-100

100 µg

Application:

ELISA

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

Biotin

Host:

Rabbit

Isotype:

IgG

Purification:

>95%, Protein G Purified

Reactivity:

Human

A52572-050

50 µg

A52572-100

100 µg

Application:

ELISA

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

FITC

Host:

Rabbit

Isotype:

IgG

Purification:

>95%, Protein G Purified

Reactivity:

Human

A52571-050

50 µg

A52571-100

100 µg

Application:

ELISA

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

HRP

Host:

Rabbit

Isotype:

IgG

Purification:

>95%, Protein G Purified

Reactivity:

Human

A52570-050

50 µg

A52570-100

100 µg

Application:

WB

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

Unconjugated

Host:

Rabbit

Isotype:

IgG

Purification:

Affinity Purified

Reactivity:

Human, Mouse

A-1004-050

50 µg

A-1004-100

100 µg

Application:

ICC, IHC

Areas of Research:

DNA Methylation

Clonality:

Monoclonal

Conjugate:

Unconjugated

Host:

Mouse

Isotype:

IgG1

Purification:

Protein G Purified

Reactivity:

Human, Mouse

A-1800-100

100 µl

Application:

IF, IP, WB

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

Unconjugated

Host:

Rabbit

Isotype:

IgG

Purification:

Affinity Purified

Reactivity:

Human

A-1005-050

50 µg

A-1005-100

100 µg

Application:

ELISA

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

Unconjugated

Host:

Rabbit

Isotype:

IgG

Purification:

>95%, Protein G Purified

Reactivity:

Escherichia coli

Trial Size Available:

Yes

A57405-020

20 µg

A57405-050

50 µg

A57405-100

100 µg

Application:

ELISA

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

Biotin

Host:

Rabbit

Isotype:

IgG

Purification:

>95%, Protein G Purified

Reactivity:

Escherichia coli

A57408-050

50 µg

A57408-100

100 µg

Application:

ELISA

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

FITC

Host:

Rabbit

Isotype:

IgG

Purification:

>95%, Protein G Purified

Reactivity:

Escherichia coli

A57407-050

50 µg

A57407-100

100 µg

Application:

ELISA

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

HRP

Host:

Rabbit

Isotype:

IgG

Purification:

>95%, Protein G Purified

Reactivity:

Escherichia coli

A57406-050

50 µg

A57406-100

100 µg

Application:

ELISA, IHC, WB

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

Unconjugated

Host:

Rabbit

Isotype:

IgG

Purification:

>95%, Protein G Purified

Reactivity:

Human, Rat

Trial Size Available:

Yes

A69019-020

20 µg

A69019-050

50 µg

A69019-100

100 µg

Application:

ELISA

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

Biotin

Host:

Rabbit

Isotype:

IgG

Purification:

>95%, Protein G Purified

Reactivity:

Human

A69022-050

50 µg

A69022-100

100 µg

Application:

ELISA

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

FITC

Host:

Rabbit

Isotype:

IgG

Purification:

>95%, Protein G Purified

Reactivity:

Human

A69021-050

50 µg

A69021-100

100 µg

Application:

ELISA

Areas of Research:

DNA Methylation

Clonality:

Polyclonal

Conjugate:

HRP

Host:

Rabbit

Isotype:

IgG

Purification:

>95%, Protein G Purified

Reactivity:

Human

A69020-050

50 µg

A69020-100

100 µg

Application:

ELISA, WB

Areas of Research: